Journal: JACC: Basic to Translational Science

Article Title: Cardiopathogenic T Cells Govern Progression and Functional Remodeling in Inflammatory Cardiomyopathy and Chronic Myocarditis

doi: 10.1016/j.jacbts.2025.101458

Figure Lengend Snippet: The Role of CD4 + T Cells in Cardiac Functional Remodeling (A) Experimental design. (B and C) Flow cytometric analysis of CD4 + T cells in peripheral blood of TCRM mice treated with IgG2b isotype control or anti-CD4 antibody. (D) Representative H&E and PSR-stained heart sections. (E) Myocarditis score and (F) quantification of PSR-positive area, in 8-week-old TCRM mice subjected to the indicated treatment. (G to J) Echocardiographic assessment of left ventricular morphology and function in 8-week-old TCRM mice treated with isotype control or anti-CD4 antibody. (G) LVID systole, (H) LVEF, (I) GCS, (J) GLS. (C, E to J) Pooled data from 3 independent experiments, including n = 12 anti-CD4-treated TCRM mice and n = 13 isotype-treated TCRM mice of which n = 5 progressed to iCMP. Statistical analysis was performed using C, unpaired 2-tailed Student’s t -test or Mann-Whitney U test and (E to J) 1-way analysis of variance with Tukey’s post hoc test or Kruskal-Wallis test with Dunnett’s multiple comparisons test. Boxplots as in . ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. Abbreviations as in and .

Article Snippet: Mice were treated with 500 μg of anti-CD4 antibody (clone: YTS191, ECACC, 87072282) or IgG2b isotype control antibody (clone: MPC-11, ECACC, ATCC CCL-167) by intraperitoneal injection twice a week as demonstrated in the respective experiments.

Techniques: Functional Assay, Control, Staining, MANN-WHITNEY

Journal: Frontiers in Cellular Neuroscience

Article Title: ACSA2 is not astrocyte-specific: implications for cell sorting strategies in the rodent brain

doi: 10.3389/fncel.2026.1756677

Figure Lengend Snippet: Intravenous antibody labeling distinguishes CNS-resident from circulating immune cells in ACSA2 + fractions. (A) Mice received I.V. injection of fluorescent antibody 3 min before tissue harvest and brain dissociation. I.V.-labeled cells represent circulating immune cells, while unlabeled CD45 + cells are CNS-resident. Created in BioRender. Daniele, E. (2026) https://BioRender.com/20anz0d . (B) Flow cytometry using anti-CD45.2-PE (I.V.) shows 20.3% of ACSA2 + cells are CD45-BV605 + /CD45.2-PE – , indicating CNS-resident immune cell contamination, while only 1.54% are circulating immune cells (CD45-BV605 + /CD45.2-PE + ). (C) Isotype control using IgG2b-κ-PE (I.V.) shows minimal non-specific labeling confirming specificity of CD45.2 labeling. Gates set on live, nucleated DRAQ5+/DAPI- singlets. Representative plots from n = 3 mice per condition.

Article Snippet: Intensity thresholds were set based on background signal measured in secondary-only and a rat IgG2b-PE isotype control (1:50, Miltenyi Biotec, Cat# 130-123-273).

Techniques: Antibody Labeling, Injection, Labeling, Flow Cytometry, Control